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fluorescent protein pnls irfp670  (Addgene inc)


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    Addgene inc fluorescent protein pnls irfp670
    Evaluation of MCF10A and 4T1 cell morphology and viability after 72 hours of culture on PDMS slabs cast from 3D printed molds coated with epoxy-acetone 0.5 mixtures, for XTC-3D epoxy (A–C) or Janchun epoxy (D–F) , compared to control PDMS cast from standard 10 cm polystyrene dishes. (A, D) Brightfield and epifluorescence images of MCF10A and 4T1 cells show comparable morphology and confluency between control and 3D printed mold conditions, supporting the biocompatibility of PDMS replicas fabricated from epoxy-coated molds. (B–C, E–F) Bar graphs quantifying viability of MCF10A cells (B, E) and 4T1 (C, F) cells cultured on control vs. 3D printed mold conditions. No significant differences (n.s.) were observed. Data represent mean ± standard deviation. Epifluorescence images in panels A and D show live MCF10A cells expressing cytoplasmic GFP (green) and nuclear RFP (magenta), and 4T1 cells expressing nuclear <t>iRFP670</t> (magenta).
    Fluorescent Protein Pnls Irfp670, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fluorescent+protein+pnls+irfp670/pNLS-iRFP670+(Plasmid+%2345466)/bio_rxiv__2025__03__29__645830-110-13-42
    Average 92 stars, based on 14 article reviews
    fluorescent protein pnls irfp670 - by Bioz Stars, 2026-09
    92/100 stars

    Images

    1) Product Images from "3D Printed Molds for Organ-on-a-Chip and Fluidics: PDMS-Based Rapid and Accessible Prototyping"

    Article Title: 3D Printed Molds for Organ-on-a-Chip and Fluidics: PDMS-Based Rapid and Accessible Prototyping

    Journal: bioRxiv

    doi: 10.1101/2025.03.29.645830

    Evaluation of MCF10A and 4T1 cell morphology and viability after 72 hours of culture on PDMS slabs cast from 3D printed molds coated with epoxy-acetone 0.5 mixtures, for XTC-3D epoxy (A–C) or Janchun epoxy (D–F) , compared to control PDMS cast from standard 10 cm polystyrene dishes. (A, D) Brightfield and epifluorescence images of MCF10A and 4T1 cells show comparable morphology and confluency between control and 3D printed mold conditions, supporting the biocompatibility of PDMS replicas fabricated from epoxy-coated molds. (B–C, E–F) Bar graphs quantifying viability of MCF10A cells (B, E) and 4T1 (C, F) cells cultured on control vs. 3D printed mold conditions. No significant differences (n.s.) were observed. Data represent mean ± standard deviation. Epifluorescence images in panels A and D show live MCF10A cells expressing cytoplasmic GFP (green) and nuclear RFP (magenta), and 4T1 cells expressing nuclear iRFP670 (magenta).
    Figure Legend Snippet: Evaluation of MCF10A and 4T1 cell morphology and viability after 72 hours of culture on PDMS slabs cast from 3D printed molds coated with epoxy-acetone 0.5 mixtures, for XTC-3D epoxy (A–C) or Janchun epoxy (D–F) , compared to control PDMS cast from standard 10 cm polystyrene dishes. (A, D) Brightfield and epifluorescence images of MCF10A and 4T1 cells show comparable morphology and confluency between control and 3D printed mold conditions, supporting the biocompatibility of PDMS replicas fabricated from epoxy-coated molds. (B–C, E–F) Bar graphs quantifying viability of MCF10A cells (B, E) and 4T1 (C, F) cells cultured on control vs. 3D printed mold conditions. No significant differences (n.s.) were observed. Data represent mean ± standard deviation. Epifluorescence images in panels A and D show live MCF10A cells expressing cytoplasmic GFP (green) and nuclear RFP (magenta), and 4T1 cells expressing nuclear iRFP670 (magenta).

    Techniques Used: Control, Cell Culture, Standard Deviation, Expressing

    Related Articles

    Stable Transfection:

    Article Title: 3D Printed Molds for Organ-on-a-Chip and Fluidics: PDMS-Based Rapid and Accessible Prototyping
    Article Snippet: 4T1 murine mammary carcinoma cells were cultured in RPMI-1640 medium with L-Glutamine (Corning) supplemented with a final concentration of 10% heat-inactivated fetal bovine serum (Gibco) and 1% gentamicin (Sigma). .. Here, we used E2 and M 4T1 clonal cell lines stably expressing near-infrared fluorescent protein (pNLS-iRFP670) and yellow fluorescent protein (YFP) in the nucleus, respectively, which we previously generated to enable live cell fluorescence imaging. pNLS-iRFP670 was a gift from Vladislav Verkhusha (Addgene plasmid # 45466; http://n2t.net/addgene:45466 ; RRID:Addgene_45466). .. The 4T1 E2 clonal cell line expressing pNLS-iRFP670 was routinely cultured in media containing 250 μg/mL G418 (Sigma) to apply selective pressure for the maintenance of pNLS-iRFP670 plasmid expression.

    Expressing:

    Article Title: 3D Printed Molds for Organ-on-a-Chip and Fluidics: PDMS-Based Rapid and Accessible Prototyping
    Article Snippet: 4T1 murine mammary carcinoma cells were cultured in RPMI-1640 medium with L-Glutamine (Corning) supplemented with a final concentration of 10% heat-inactivated fetal bovine serum (Gibco) and 1% gentamicin (Sigma). .. Here, we used E2 and M 4T1 clonal cell lines stably expressing near-infrared fluorescent protein (pNLS-iRFP670) and yellow fluorescent protein (YFP) in the nucleus, respectively, which we previously generated to enable live cell fluorescence imaging. pNLS-iRFP670 was a gift from Vladislav Verkhusha (Addgene plasmid # 45466; http://n2t.net/addgene:45466 ; RRID:Addgene_45466). .. The 4T1 E2 clonal cell line expressing pNLS-iRFP670 was routinely cultured in media containing 250 μg/mL G418 (Sigma) to apply selective pressure for the maintenance of pNLS-iRFP670 plasmid expression.

    Generated:

    Article Title: 3D Printed Molds for Organ-on-a-Chip and Fluidics: PDMS-Based Rapid and Accessible Prototyping
    Article Snippet: 4T1 murine mammary carcinoma cells were cultured in RPMI-1640 medium with L-Glutamine (Corning) supplemented with a final concentration of 10% heat-inactivated fetal bovine serum (Gibco) and 1% gentamicin (Sigma). .. Here, we used E2 and M 4T1 clonal cell lines stably expressing near-infrared fluorescent protein (pNLS-iRFP670) and yellow fluorescent protein (YFP) in the nucleus, respectively, which we previously generated to enable live cell fluorescence imaging. pNLS-iRFP670 was a gift from Vladislav Verkhusha (Addgene plasmid # 45466; http://n2t.net/addgene:45466 ; RRID:Addgene_45466). .. The 4T1 E2 clonal cell line expressing pNLS-iRFP670 was routinely cultured in media containing 250 μg/mL G418 (Sigma) to apply selective pressure for the maintenance of pNLS-iRFP670 plasmid expression.

    Fluorescence:

    Article Title: 3D Printed Molds for Organ-on-a-Chip and Fluidics: PDMS-Based Rapid and Accessible Prototyping
    Article Snippet: 4T1 murine mammary carcinoma cells were cultured in RPMI-1640 medium with L-Glutamine (Corning) supplemented with a final concentration of 10% heat-inactivated fetal bovine serum (Gibco) and 1% gentamicin (Sigma). .. Here, we used E2 and M 4T1 clonal cell lines stably expressing near-infrared fluorescent protein (pNLS-iRFP670) and yellow fluorescent protein (YFP) in the nucleus, respectively, which we previously generated to enable live cell fluorescence imaging. pNLS-iRFP670 was a gift from Vladislav Verkhusha (Addgene plasmid # 45466; http://n2t.net/addgene:45466 ; RRID:Addgene_45466). .. The 4T1 E2 clonal cell line expressing pNLS-iRFP670 was routinely cultured in media containing 250 μg/mL G418 (Sigma) to apply selective pressure for the maintenance of pNLS-iRFP670 plasmid expression.

    Imaging:

    Article Title: 3D Printed Molds for Organ-on-a-Chip and Fluidics: PDMS-Based Rapid and Accessible Prototyping
    Article Snippet: 4T1 murine mammary carcinoma cells were cultured in RPMI-1640 medium with L-Glutamine (Corning) supplemented with a final concentration of 10% heat-inactivated fetal bovine serum (Gibco) and 1% gentamicin (Sigma). .. Here, we used E2 and M 4T1 clonal cell lines stably expressing near-infrared fluorescent protein (pNLS-iRFP670) and yellow fluorescent protein (YFP) in the nucleus, respectively, which we previously generated to enable live cell fluorescence imaging. pNLS-iRFP670 was a gift from Vladislav Verkhusha (Addgene plasmid # 45466; http://n2t.net/addgene:45466 ; RRID:Addgene_45466). .. The 4T1 E2 clonal cell line expressing pNLS-iRFP670 was routinely cultured in media containing 250 μg/mL G418 (Sigma) to apply selective pressure for the maintenance of pNLS-iRFP670 plasmid expression.

    Plasmid Preparation:

    Article Title: 3D Printed Molds for Organ-on-a-Chip and Fluidics: PDMS-Based Rapid and Accessible Prototyping
    Article Snippet: 4T1 murine mammary carcinoma cells were cultured in RPMI-1640 medium with L-Glutamine (Corning) supplemented with a final concentration of 10% heat-inactivated fetal bovine serum (Gibco) and 1% gentamicin (Sigma). .. Here, we used E2 and M 4T1 clonal cell lines stably expressing near-infrared fluorescent protein (pNLS-iRFP670) and yellow fluorescent protein (YFP) in the nucleus, respectively, which we previously generated to enable live cell fluorescence imaging. pNLS-iRFP670 was a gift from Vladislav Verkhusha (Addgene plasmid # 45466; http://n2t.net/addgene:45466 ; RRID:Addgene_45466). .. The 4T1 E2 clonal cell line expressing pNLS-iRFP670 was routinely cultured in media containing 250 μg/mL G418 (Sigma) to apply selective pressure for the maintenance of pNLS-iRFP670 plasmid expression.



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    fluorescent protein pnls irfp670  (Addgene inc)
    92
    Addgene inc fluorescent protein pnls irfp670
    Evaluation of MCF10A and 4T1 cell morphology and viability after 72 hours of culture on PDMS slabs cast from 3D printed molds coated with epoxy-acetone 0.5 mixtures, for XTC-3D epoxy (A–C) or Janchun epoxy (D–F) , compared to control PDMS cast from standard 10 cm polystyrene dishes. (A, D) Brightfield and epifluorescence images of MCF10A and 4T1 cells show comparable morphology and confluency between control and 3D printed mold conditions, supporting the biocompatibility of PDMS replicas fabricated from epoxy-coated molds. (B–C, E–F) Bar graphs quantifying viability of MCF10A cells (B, E) and 4T1 (C, F) cells cultured on control vs. 3D printed mold conditions. No significant differences (n.s.) were observed. Data represent mean ± standard deviation. Epifluorescence images in panels A and D show live MCF10A cells expressing cytoplasmic GFP (green) and nuclear RFP (magenta), and 4T1 cells expressing nuclear <t>iRFP670</t> (magenta).
    Fluorescent Protein Pnls Irfp670, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fluorescent+protein+pnls+irfp670/pNLS-iRFP670+(Plasmid+%2345466)/bio_rxiv__2025__03__29__645830-110-13-42
    Average 92 stars, based on 1 article reviews
    fluorescent protein pnls irfp670 - by Bioz Stars, 2026-09
    92/100 stars
    		  Buy from Supplier
    fluorescent protein  (Addgene inc)
    92
    Addgene inc fluorescent protein
    Figure 3. Depletion of RanBP1 using an Auxin-induced degron (AID) system. (a) A schematic diagram illustrating AID system. (b) Western blotting with anti-RanBP1 antibodies of parental HCT116 (control, left lane) and HCT116RanBP1-μAID/HA cells that were incubated without (middle lane) or with (right lane) 1 mM Auxin for 3 h. Note efficient degradation of RanBP1-µAID-HA upon Auxin addition. Actin is shown as a loading control (lower panel). (c) Images of DLD1RanBP1-Neon/3mAID/FLAG cells, in which both endogenous RanBP1 and RCC1 are tagged (RanBP1 is tagged with <t>fluorescent</t> protein (Neon), three copies of a minimal AID (3 x mini-AID) and a FLAG tag (RanBP1-Neon-3mAID-FLAG) and RCC1 is tagged with infrared fluorescent protein: <t>RCC1-iRFP670::TIR1).</t> Cells are shown before (left column) and after (right column) addition of 1 mM Auxin for 3 h. Note localization of RanBP1 to mitotic spindle. Scale bars = 10 μm.
    Fluorescent Protein, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fluorescent+protein+pnls+irfp670/pNLS-iRFP670+(Plasmid+%2345466)/pm32594833-54-21-24
    Average 92 stars, based on 1 article reviews
    fluorescent protein - by Bioz Stars, 2026-09
    92/100 stars
    		  Buy from Supplier

    Image Search Results


    Evaluation of MCF10A and 4T1 cell morphology and viability after 72 hours of culture on PDMS slabs cast from 3D printed molds coated with epoxy-acetone 0.5 mixtures, for XTC-3D epoxy (A–C) or Janchun epoxy (D–F) , compared to control PDMS cast from standard 10 cm polystyrene dishes. (A, D) Brightfield and epifluorescence images of MCF10A and 4T1 cells show comparable morphology and confluency between control and 3D printed mold conditions, supporting the biocompatibility of PDMS replicas fabricated from epoxy-coated molds. (B–C, E–F) Bar graphs quantifying viability of MCF10A cells (B, E) and 4T1 (C, F) cells cultured on control vs. 3D printed mold conditions. No significant differences (n.s.) were observed. Data represent mean ± standard deviation. Epifluorescence images in panels A and D show live MCF10A cells expressing cytoplasmic GFP (green) and nuclear RFP (magenta), and 4T1 cells expressing nuclear iRFP670 (magenta).

    Journal: bioRxiv

    Article Title: 3D Printed Molds for Organ-on-a-Chip and Fluidics: PDMS-Based Rapid and Accessible Prototyping

    doi: 10.1101/2025.03.29.645830

    Figure Lengend Snippet: Evaluation of MCF10A and 4T1 cell morphology and viability after 72 hours of culture on PDMS slabs cast from 3D printed molds coated with epoxy-acetone 0.5 mixtures, for XTC-3D epoxy (A–C) or Janchun epoxy (D–F) , compared to control PDMS cast from standard 10 cm polystyrene dishes. (A, D) Brightfield and epifluorescence images of MCF10A and 4T1 cells show comparable morphology and confluency between control and 3D printed mold conditions, supporting the biocompatibility of PDMS replicas fabricated from epoxy-coated molds. (B–C, E–F) Bar graphs quantifying viability of MCF10A cells (B, E) and 4T1 (C, F) cells cultured on control vs. 3D printed mold conditions. No significant differences (n.s.) were observed. Data represent mean ± standard deviation. Epifluorescence images in panels A and D show live MCF10A cells expressing cytoplasmic GFP (green) and nuclear RFP (magenta), and 4T1 cells expressing nuclear iRFP670 (magenta).

    Article Snippet: Here, we used E2 and M 4T1 clonal cell lines stably expressing near-infrared fluorescent protein (pNLS-iRFP670) and yellow fluorescent protein (YFP) in the nucleus, respectively, which we previously generated to enable live cell fluorescence imaging. pNLS-iRFP670 was a gift from Vladislav Verkhusha (Addgene plasmid # 45466; http://n2t.net/addgene:45466 ; RRID:Addgene_45466).

    Techniques: Control, Cell Culture, Standard Deviation, Expressing

    Figure 3. Depletion of RanBP1 using an Auxin-induced degron (AID) system. (a) A schematic diagram illustrating AID system. (b) Western blotting with anti-RanBP1 antibodies of parental HCT116 (control, left lane) and HCT116RanBP1-μAID/HA cells that were incubated without (middle lane) or with (right lane) 1 mM Auxin for 3 h. Note efficient degradation of RanBP1-µAID-HA upon Auxin addition. Actin is shown as a loading control (lower panel). (c) Images of DLD1RanBP1-Neon/3mAID/FLAG cells, in which both endogenous RanBP1 and RCC1 are tagged (RanBP1 is tagged with fluorescent protein (Neon), three copies of a minimal AID (3 x mini-AID) and a FLAG tag (RanBP1-Neon-3mAID-FLAG) and RCC1 is tagged with infrared fluorescent protein: RCC1-iRFP670::TIR1). Cells are shown before (left column) and after (right column) addition of 1 mM Auxin for 3 h. Note localization of RanBP1 to mitotic spindle. Scale bars = 10 μm.

    Journal: Cell cycle (Georgetown, Tex.)

    Article Title: RanBP1 controls the Ran pathway in mammalian cells through regulation of mitotic RCC1 dynamics.

    doi: 10.1080/15384101.2020.1782036

    Figure Lengend Snippet: Figure 3. Depletion of RanBP1 using an Auxin-induced degron (AID) system. (a) A schematic diagram illustrating AID system. (b) Western blotting with anti-RanBP1 antibodies of parental HCT116 (control, left lane) and HCT116RanBP1-μAID/HA cells that were incubated without (middle lane) or with (right lane) 1 mM Auxin for 3 h. Note efficient degradation of RanBP1-µAID-HA upon Auxin addition. Actin is shown as a loading control (lower panel). (c) Images of DLD1RanBP1-Neon/3mAID/FLAG cells, in which both endogenous RanBP1 and RCC1 are tagged (RanBP1 is tagged with fluorescent protein (Neon), three copies of a minimal AID (3 x mini-AID) and a FLAG tag (RanBP1-Neon-3mAID-FLAG) and RCC1 is tagged with infrared fluorescent protein: RCC1-iRFP670::TIR1). Cells are shown before (left column) and after (right column) addition of 1 mM Auxin for 3 h. Note localization of RanBP1 to mitotic spindle. Scale bars = 10 μm.

    Article Snippet: To establish HCT116RanBP1-μAID and HCT116RanGAP1−3mAID cell lines, we first used CRISPR/Cas9 to tag the C-terminus of RCC1 with sequences encoding infra-red fluorescent protein (iRFP670, Addgene #45466) and TIR1-9Myc, separated by a P2A sequence.

    Techniques: Western Blot, Control, Incubation, FLAG-tag

    Figure 4. RanBP1 is dispensable for cell growth and nucleo-cytoplasmic transport of a model substrate. (a) Crystal violet assay of HCT116RanBP1-μAID/HA and parental HCT116 cells grown with or without Auxin (1 mM) for 4 d. Quantification of the number of cells are shown on the right. (b) Left: HCT116RanBP1-μAID/HA cells, expressing a model NLS-NES-containing substrate (NLS-mCherry-LEXY). In this system, exposure of cells to UV light induces nuclear export, while switching off light induces nuclear import. White circle indicates the region of fluorescent intensity measurements. Right: Quantification of nucleocytoplasmic transport in HCT116RanBP1-

    Journal: Cell cycle (Georgetown, Tex.)

    Article Title: RanBP1 controls the Ran pathway in mammalian cells through regulation of mitotic RCC1 dynamics.

    doi: 10.1080/15384101.2020.1782036

    Figure Lengend Snippet: Figure 4. RanBP1 is dispensable for cell growth and nucleo-cytoplasmic transport of a model substrate. (a) Crystal violet assay of HCT116RanBP1-μAID/HA and parental HCT116 cells grown with or without Auxin (1 mM) for 4 d. Quantification of the number of cells are shown on the right. (b) Left: HCT116RanBP1-μAID/HA cells, expressing a model NLS-NES-containing substrate (NLS-mCherry-LEXY). In this system, exposure of cells to UV light induces nuclear export, while switching off light induces nuclear import. White circle indicates the region of fluorescent intensity measurements. Right: Quantification of nucleocytoplasmic transport in HCT116RanBP1-

    Article Snippet: To establish HCT116RanBP1-μAID and HCT116RanGAP1−3mAID cell lines, we first used CRISPR/Cas9 to tag the C-terminus of RCC1 with sequences encoding infra-red fluorescent protein (iRFP670, Addgene #45466) and TIR1-9Myc, separated by a P2A sequence.

    Techniques: Crystal Violet Assay, Expressing